Antibody Screening for Secreted Proteins Expressed in Pichia pastoris

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Antibody screening for secreted proteins expressed in Pichia pastoris.

genes. Methods Enzymol. 185:60-89. 5.Switzer, R.C., C.R. Merril and S. Shifrin. 1979. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal. Biochem. 98:231-237. 6.Tabor, S. and C.C. Richardson. 1985. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82:1074-1078. 7.Whela...

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Members of the six-transmembrane segment family of ion channels share a common structural design. However, there are sequence differences between the members that confer distinct biophysical properties on individual channels. Currently, we do not have 3D structures for all members of the family to help explain the molecular basis for the differences in their biophysical properties and pharmacol...

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Phospholipase C from genetically modified Pichia pastoris (CTA) 2008 - Page 1(7) PHOSPHOLIPASE C EXPRESSED IN PICHIA PASTORIS

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Protein expression in the microbial eukaryotic host Pichia pastoris offers the possibility to generate high amounts of recombinant protein in a fast and easy to use expression system. As a single-celled microorganism P. pastoris is easy to manipulate and grows rapidly on inexpensive media at high cell densities. Being a eukaryote, P. pastoris is able to perform many of the post-translational mo...

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ژورنال

عنوان ژورنال: BioTechniques

سال: 1996

ISSN: 0736-6205,1940-9818

DOI: 10.2144/96215bm11